FAQs

Because you most likely have not completed your order here.

  1. Identify whether you want a cell line to grow in tissue culture, or one to grow in the brain of a mouse.
  2. Put your intended cell lines in the cart.
  3. Proceed to checkout.
  4. Put in your name, phone number, email address and postal address.
  5. In the order notes put your own FedEx number if you don’t want to be overly charged for shipping.
  6. Read the terms and conditions and know that you are not to share these with others unless they work under you etc.
  7. Press the “place order” button. This provides a quote for your company and reserves the stock for you.
  8. We email you a confirmation and will send any information your company needs to set us up as vendors etc.
  9. Research Cell Bank (RCB) a division of Fred Hutch that is outside of the Olson Lab sends the vials, and processes a payment upon arrival.

YOU DO NOT PROVIDE ANY PAYMENT INFORMATION ON THIS SITE, YOU CAN NOT GET YOUR CELLS UNTIL YOU HAVE PROCESSED AN ORDER HERE.

Why? Because the terms and conditions serves as the MTA between the Hutch and you. Until that is done, no vials leave the Hutch.

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Very few of our medulloblastomas have been successful in culture, and they have a reputation for being very difficult to be grown in this way. Under no circumstances do we advise putting our orthotopic PDX cells into TC. If you are interested in growing these cell lines in TC please purchase those lines and note our return policy does not include the use of our orthotopic PDX cell lines in TC.

PRIMARY CELL LINE TISSUE CULTURE 8.20.15.pdf

Why is it necessary to seed the cell in a laminin coated dish? Are they not able to grow in suspension? Do the form spheres?

Laminin coating was done in the protocol we follow.

But we are not just blindly following the protocol. Most of our lines die if they do not have laminin coated plates, so it is necessary. A couple of our lines can grow as clumps or spheres in suspension but that is not ideal.

Useful cell lines that grow in suspension tend to float around as single cells. Clumps or spheres of cells are much harder to work with. You have to disperse the cells before you do anything and that is not as easy as it sounds.

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–       The best way to expand the PDX mouse derived line is by orthotopic xenograft. One vial can easily supply enough material for implantation into 5-10 mice, and a single tumor grown in mice can supply many more than that. Expansion in this way will provide enough material for experiments in tissue culture or in flank xenografts, as well as passaging in mice to keep the model going.

–       A vial probably does not contain enough cells to create a flank xenograft. Similar to expanding in culture conditions our tumor models only grown in the microenvironment of the brain, and growing these as flank tumors may fundamentally change the model.

–       A vial may contain enough cells to begin a cell culture, but our models have never been exposed to culture conditions which might change the behavior of this model. Human tumors are also have a high failure rate in culture and our lab will not supply vials to people who put our mouse lines into TC. Under no circumstances do we advise putting our orthotopic PDX cells into TC. If you are interested in growing these cell lines in TC please purchase those lines and note that our return policy does not include the use of our orthotopic PDX mouse cell lines in TC.

Here are links to our current SOPs in PDF version:

Preparing Cryopreserved Cell Suspension for Transplant.pdf

PDX Surgery.pdf

All of our tumor models were prepared from a human patient tumor sample that never saw culture conditions and were grown exclusively as orthotopic xenograft in mice.

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Tumor onset can range anywhere from 3 weeks to 6 months depending on the tumor model. Tumor cells coming out of frozen sometimes take a little longer to form observable tumors in mice, but once a tumor is collected and passaged the time to onset should decrease slightly and become more predictable.

Generalized tumor onset for each line here:

olson-lab-line-info-10-3-16-btrl-org.xlsx

For more specific tumor onset time, please contact us with your exact cell line and passage information!

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These lines tested:

Med-513 FH
Atrt-333 FH
Med-1512 FH
Med-211 FH
Med-113 FH

PDF here

Gbm-311FH

Med-114FH

Gbm-711FH

Astro-412FH

Astro-110FH

Bsg-211(A)FH

Neuro-211FH

Epd-210FH

Epd-410FH

Med-2312FH

Med-610FH

Atrt-312FH

Med-1911FH

Med-2112FH

Med-311FH

Med-813FH

Med-512FH

Med-1712FH

Med-411FH

Med-511FH

IDEXX_Case_29677-2014

 

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Records of the mycoplasma assay results:

Copy of Myco Results Wise 6.8.2015

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–       We currently use our NSG breeding colony originally started from NSG mice from Jax. We have used Nu/Nu mice as well but have moved completely to NSG because of their greater degree of immunosuppression and ease of maintaining a breeding colony.

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We inject 100,000 cells in a volume of 2ul per mouse.

Here are links to our current SOPs in PDF version:

Preparing Cryopreserved Cell Suspension for Transplant.pdf

PDX Surgery.pdf

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Under these conditions we offer a replacement or refund:

If your cells arrive with less than 15% viability.

The fidelity of the human cell line can not be verified for the passage we sent you.

Is it months from average tumor onset and you still don’t have tumors?

Contact us for troubleshooting

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All listed prices are for academia. Industry queries should be addressed to the office of Industry Relations and Technology Transfer:

Fred Hutchinson Cancer Research Center
Industry Relations and Technology Transfer Office
P.O. Box 19024, J2-110
Seattle, WA 98109-1024

Location
823 Yale Ave. N.
Seattle, WA 98109

For general inquiries, contact:
Email: IRTTO@fhcrc.org
Phone:
Fax: (206) 667-4732
Mail stop: J2-110

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Using your own FedEx account # will save you money! It is a minimum of 60$ if we have to use our own to send them out!

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At this time we are not offering back-ordering of cell lines as tumor onset can be quite long for several of our lines. Please use the alert system to be notified by email when a sample becomes available (it is where the “place in cart” button would be if it were in stock).

Thank you!

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